For me, it is a big difference. First there is just the muscle memory of the feel of the fret spacing. I have talked to different guitarists, lots of guys jump around from Strats to Les Pauls to PRS all the time, so they learn to lock in quickly to whatever scale length they are playing at the time. My brain does not seem to work that way. In the last four years as I have gotten back into guitar, I have played mostly the 25" and 24.5" scale. I can get used to either, but I always seem to come back to preferring the 24.5" scale, both in feel and in tone.
Also for me it is the slinkiness in the bends. I am not a great lead player, but I do like to noodle around quite a bit with classic rock and blues leads. With a 24.5" scale, 10's are perfect for me, nice and slinky. I can even handle 11's, especially when dropped a half step. With 25" guitars, I prefer 9.5's over 10's re bends and vibrato, but I like the thicker feel of 10's better. Also I seem to have the bends more naturally "locked in" to my brain for 24.5" scale, I seem to intuitively know where to bend to for full step, one and a half step and even two step bends, I just seem to know where to go, I can hit it spot on even with a vibrato. But with the 25" scale, it is not as intuitive for me, I have to consciously think about it more and can miss it and have to adjust quickly on the fly.
Thus, 24.5" is home for me!
P.S. Pattern Regular - I do not like. My Swamp Ash Studio, I dig the Stratty tones, only dislike is the Pattern Regular Carve. My favorite is Pattern, followed by Wide Fat.
I know I am really picky and bothered by things that are not exactly as I like them. I am that way with just about everything! I like what I like, and focus in on that. Thus the narrow collection, no Gibson, no Fender, just a bunch of awesome PRS Private 245's, for the most part!
